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PHYSIOLOGIC EVIDENCES OF GOOD TOLERANCE OF CONCURRENT RUMEN FISTULATION AND DUODENAL CANNULATION IN WEST AFRICAN DWARF SHEEP

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Abstract

Twelve adult West African Dwarf (WAD) sheep of mean age and body weight of 18 ± 1.19 months and 14.69 ± 256 kg body weight respectively were used to study the compatibility of concurrent rumen fisulation and duodenal cannulation with normal life. The compatibility with norma life was assessed by differences in body weight, voluntary feed intake, rectal temperature and some haematological and biochemical parameters for a period of eight weeks between test and contro groups. During the sudyperiod, all the six animals survived the surgery. There was no significant (p < 0.05) dfference in body weight between the test and control groups for the entire study period (week 1-8). The mean rectal temperature of the test group was significantly (p < 0.05) higher than the control group on week 1(44.6 ± 3.150C), week 2 (43.26 ± 414 0C) and week 3 (41.15 ± 6620C) after implantation. Packed cell volume (PCV) and erythrocyte count (EC) were significantly reduced in the test group within the frst three weeks (33.19 ± 141 to 24.45 ± 1.39%and 7.01 ± 134 to 437 ± 1.58 x 106μl) respectively. On the contrary, total leukocyte count (TLC), and neutrophils were significantly higher in the test group between weeks 1 to 3 (12.48 ± 435 and 31.09 ± 3.67 respectvely) compared to the control (8.06 ± 0.95 and 23.41 ± 2.09 respecvely. Lymphocytes were insignificantly higher in the test group while voluntary feed intake was insigniicantly reduced in the test group throughout the eight weeks. Biochemical analyses revealed that serum creatinine was signicantly higher in the test group at week 1 (3.62 ± 1.18) and week 2 (3.08 ± 1.44) afer surgery. There was however, no significant difference in serum total proteins during the study period. Serum potassium concentrations were sgniicantly decreased inthe test group at week 1 (4.96 ± 1.03 to 1.34 ± 0.04). Sodum was also signcanty reduced (146.08 ± 3.78 to 96.03 ± 6.21) but on week 3 post impantation (PI). On the other hand, serum globulin was significantly higher in the test group throughout the study period. Gross observationsof incision sites showed adhesions characterized by coalesced tissue granulations. The study has shown that the use of polymetric materials in fabricating rumen fistulae and duodenal cannuale could pave way for increased routine nutritional studies in ruminants particularly in developing countries where the availability of these materials constitute great limitationto research involving rumen studies.