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SCREENING OF THREE STANDARD BASAL MEDIA FOR IN VITRO PROPAGATION OF GLYCINE MAX LINN. ZYGOTIC EMBRYOS

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Abstract

Three different standard basal media were screened for the propagation of Glycine max Linn. zygotic embryos in vitro. The media employed were those of Murashige and Skoog (MS) (1962), Gamborg et al. (B5) (1968) and Schenk and Hildebrandt (SH) (1972). This study was done to determine the most suitable medium for the propagation of G. max Linn. zygotic embryos. The results obtained showed that although all the three basal media screened supported the in vitro propagation of the embryos but MS medium was the most effective in this respect. The embryos with the longest shoot (10.98 ± 1.00 cm), longest root (5.5 ± 0.59 cm) and highest fresh weights (0.2682 ± 1.00 g) were observed among those cultured in the MS medium whereas the B5 medium produced embryos with the highest percentage sprout (28.57 ± 0.51 %) and SH medium produced the highest sprout rate (0.4575 ± 0.384). The embryos were able to develop into normal plantlets even in the absence of externally applied plant growth regulators. This may suggest the presence of endogenous hormone in the zygotic embryos at a level that supported the propagation of the embryos. Also, the levels of ions present in all the media would have been optimum as plantlets which grew into normal seedlings were regenerated in all of them. This work recommends the use of MS medium for the propagation of G.max Linn. zygotic embryos as it proved to be the most effective of the three media screened.